Journal: The British Journal of Dermatology

Article Title: Does the gene matter? Genotype–phenotype and genotype–outcome associations in congenital melanocytic naevi [Link]

doi: 10.1111/bjd.18106

Figure Lengend Snippet: Summary of genotype and phenotype of cohort

Article Snippet: Cells were incubated overnight at 4 °C with BRAF V600E antibody 1 : 1000 (ab200535; Abcam, Cambridge, U.K.), with appropriate negative controls, followed by incubation with goat anti‐rabbit IgG‐conjugated Alexa Fluor 488 secondary antibody (A‐11034; Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: Mutagenesis, Variant Assay

Journal: The British Journal of Dermatology

Article Title: Does the gene matter? Genotype–phenotype and genotype–outcome associations in congenital melanocytic naevi [Link]

doi: 10.1111/bjd.18106

Figure Lengend Snippet: Sanger sequencing trace of DNA extracted directly from (a) congenital melanocytic naevus ( CMN ) tissue (upper) and DNA extracted from cultured naevus cells (lower) from the same patient, demonstrating BRAF c.1799T>A, p.(V600E) in both, which appears low‐level somatic in whole tissue and heterozygous in naevus cells. (b) A similar phenomenon is observed in naevus cells cultured directly from a patient with NRAS c183A>G, p.(Q61R). Immunocytochemistry of BRAF p.(V600E) naevus cells shows pancytoplasmic uniform expression of BRAF V600E (green) in all naevus cells. Cells are counterstained with Hoechst stain (blue) and Alexa Fluor 647‐conjugated phalloidin antibody (red). BRAF V600E negative control at original magnification × 20 (c), and BRAF V600E immunostaining at × 20 (d) and × 63 (e).

Article Snippet: Cells were incubated overnight at 4 °C with BRAF V600E antibody 1 : 1000 (ab200535; Abcam, Cambridge, U.K.), with appropriate negative controls, followed by incubation with goat anti‐rabbit IgG‐conjugated Alexa Fluor 488 secondary antibody (A‐11034; Thermo Fisher Scientific) for 1 h at room temperature.

Techniques: Sequencing, Cell Culture, Immunocytochemistry, Expressing, Staining, Negative Control, Immunostaining

Journal: Frontiers in Endocrinology

Article Title: The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients

doi: 10.3389/fendo.2022.970682

Figure Lengend Snippet: Characteristics of the radiogenic and sporadic papillary thyroid microcarcinomas in patients aged up to 30 years.

Article Snippet: In brief, a mouse monoclonal anti-BRAF (mutated V600E) antibody (VE1) ab228461(Abcam) at a 1:100 dilution and the Novolink Polymer Detection System (250T) (Leica RE7140-K) were used to detect IHC reaction product.

Techniques: Labeling, Dissection, Activity Assay

Journal: Frontiers in Endocrinology

Article Title: The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients

doi: 10.3389/fendo.2022.970682

Figure Lengend Snippet: Characteristics of the radiogenic and sporadic papillary thyroid microcarcinomas in pediatric patients aged ≤ 18 years.

Article Snippet: In brief, a mouse monoclonal anti-BRAF (mutated V600E) antibody (VE1) ab228461(Abcam) at a 1:100 dilution and the Novolink Polymer Detection System (250T) (Leica RE7140-K) were used to detect IHC reaction product.

Techniques: Labeling, Dissection, Activity Assay

Journal: Frontiers in Endocrinology

Article Title: The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients

doi: 10.3389/fendo.2022.970682

Figure Lengend Snippet: Characteristics of the radiogenic and sporadic papillary thyroid microcarcinomas in young adult patients aged 19-30 years.

Article Snippet: In brief, a mouse monoclonal anti-BRAF (mutated V600E) antibody (VE1) ab228461(Abcam) at a 1:100 dilution and the Novolink Polymer Detection System (250T) (Leica RE7140-K) were used to detect IHC reaction product.

Techniques: Labeling, Dissection, Activity Assay

Journal: Frontiers in Endocrinology

Article Title: The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients

doi: 10.3389/fendo.2022.970682

Figure Lengend Snippet: Characteristics of the radiogenic and sporadic papillary thyroid microcarcinomas sized up to 5 mm.

Article Snippet: In brief, a mouse monoclonal anti-BRAF (mutated V600E) antibody (VE1) ab228461(Abcam) at a 1:100 dilution and the Novolink Polymer Detection System (250T) (Leica RE7140-K) were used to detect IHC reaction product.

Techniques: Labeling, Dissection, Activity Assay

Journal: Frontiers in Endocrinology

Article Title: The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients

doi: 10.3389/fendo.2022.970682

Figure Lengend Snippet: Sporadic recurrent MPTC sized 8 mm (pT1aN0M0 at the first operation) removed from a 22-year-old male patient: (A–D) primary tumor, (E–H) the RAI-R recurrent metastasis. (A) Nonencapsulated primary tumor with follicular-papillary (conventional) growth pattern with extrathyroidal extension to the connective tissue, H&E, 15X magnification. (B) Fragment of the primary tumor with papillary structures featuring tall cell areas and oncocytic changes, H&E, 400X magnification. (C) Primary tumor: positive IHC reaction with the anti-BRAF (mutated V600E) antibody, 400X magnification. (D) Primary tumor: IHC reaction with Ki67 (Clone MIB-1) antibody (Ki67 LI 3.1%), 400X magnification. (E) RAI-R recurrent metastasis with cystic changes removed 1.3 years after the first surgery, H&E, 15X magnification. (F) Fragment of the RAI-R recurrent metastasis with solid structure and oncocytic changes, H&E, 400X magnification. (G) fragment of the RAI-R recurrent metastasis: positive IHC reaction with anti-BRAF (mutated V600E) antibody, 400X magnification. (H) RAI-R recurrent metastasis: IHC reaction with Ki67 (Clone MIB-1) antibody (Ki67 LI 3.3%), 400X magnification.

Article Snippet: In brief, a mouse monoclonal anti-BRAF (mutated V600E) antibody (VE1) ab228461(Abcam) at a 1:100 dilution and the Novolink Polymer Detection System (250T) (Leica RE7140-K) were used to detect IHC reaction product.

Techniques: